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CustomArray 12K FAQ
CustomArray 4X2K FAQ
Hyb & Seq FAQ
Stripping Kit FAQ
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Protocols & Docs

These protocols describe how to hybridize and image CustomArray® chips. Methods for preparing the labeled nucleic acid sample are the responsibility of the user.

The recommended hybridization conditions can be varied to suit the application. However, CombiMatrix has optimized this protocol extensively to work on the CustomArray® system.

To save the file, please right click the link and select "Save Target As." All files are in .pdf format. You must have Adobe Acrobat to view the file.

FLUORESCENT DETECTION METHOD
Application Protocol
Comparitive Genome Hybridization,
Genome Tiling

Whole Genome Amplification - Preparation of Biotinylated DNA Target Samples (PTL015)

Comparative Genome Hybridization Using CustomArray® 12K (PTL004)

Gene Expression

CustomArray® 12K Microarray - Hybridization and Imaging (PTL006)

CustomArray® 4X2K Microarray - Hybridization and Imaging (PTL005)

CustomArray® 90K Microarray - Hybridization and Imaging Protocol (PTL020)

MicroRNA Analysis

Preparation of Labeled MicroRNA Samples for Hybridization with 4X2K Microarrays (PTL013)

MicroRNA 4x2K Microarray - Hybridization and Imaging (PTL012)

Pathogen Typing

Influenza A Microarray 12K - Target Preparation, Hybridization, and Imaging (PTL011)

Influenza A Microarray 4X2K - Target Preparation, Hybridization, and Imaging (PTL010)

Re-sequencing and SNP Detection

Re-sequencing and SNP Detection Using CustomArray® 12K (PTL014)

Hyb & Seq™ Protocol for SNP Detection and Re-sequencing Using CustomArray® 12K Microarrays (PTL009)

Stripping of CombiMatrix Microarrays

Stripping and Re-hybridization of CombiMatrix 12K Microarray (PTL001)

Stripping and Re-hybridization of CombiMatrix 4X2K Microarray (PTL002)

Stripping and Preparation of CombiMatrix 90K Microarrays for Re-hybridization (PTL022)


ELECTRASENSE® DETECTION METHOD
Application Protocol
Gene Expression ElectraSense® 12K/4x2K Microarray - Hybridization and Electrochemical Detection (PTL021)
Pathogen Typing ElectraSense® Influenza A 12K Microarray - Target Preparation, Hybridization, and Electrochemical Detection (PTL016)
Stripping of ElectraSense® Microarrays Stripping and Re-hybridization of ElectraSense® 12K Microarrays (PTL003)

softwaremanual

ELECTRASENSE®
Product Manual
ElectraSense® ElectraSense® Reader and Software Manual (PTL017)

appnote

CustomArray Stripping Kit Application Note - Performance of CustomArrays® after stripping and re-hybridization


Click on link below to view the technical data article in .pdf format. To save the file, please right click the link and select "Save Target As".

"Iterative development of microarray probes for accessing the toxicity of a novel class of chemical entities". White paper on CustomArray® 4X2K

"Performance of 12000 feature oligonucleotide microarray manufactured by in situ electrochemical synthesis of DNA"

"Assessing the sensitivity, dynamic range and reproducibility of the CombiMatrix CustomArray® platform" - R. Orbus, J. Cooper, M. Strathmann

       

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