CombiMatrix has created the first commercially
available microarray stripping kit. The kit allows researchers to strip
the target from a recently hybridized CustomArray™ enabling a
subsequent re-hybridization. With the Stripping Kit, each CustomArray™
can be stripped and re-hybridized three times, yielding a total of four
hybridizations and images per CustomArray™. The ability to re-use
CustomArray™ microarrays reduces the cost per CustomArray™ to the
researcher while eliminating problems associated with chip-to-chip
reproducibility.
Figure 1. Images of a
bottom sector of a CustomArray™
4x2K microarray before (A) and after (B) stripping.
The stripping procedure is based on denaturation
of DNA:DNA or DNA:RNA hybrids. This denaturation results in labeled
targets being removed from the oligonucleotide probes and subsequently
washed off the CustomArray™ while the original probes remain. The
stripped CustomArray™ can then be re-hybridized with a new target
sample and re-imaged.
CombiMatrix does not recommend increasing the
number of re-uses beyond the recommended three (total of four
hybridizations). Tests in our lab show that a CustomArray™ can be
stripped up to three times without significantly decreasing the
microarray quality.
CustomArray™ Stripping Kit Benefits
Conduct more tests per CustomArray™
Decrease actual cost per CustomArray™
Eliminate technical errors due to chip-to-chip
reproducibility
Simple protocol washes away target while
probes remain
Strip and re-image your CustomArray™ up to
three times
Contents of CustomArray™ Stripping
Kit
One CustomArray™ Stripping Kit is intended for
25 uses
25 mL of CustomArray™ Stripping Solution
10 mL of CustomArray™ Imaging Solution
1 stripping cap (multiple use)
25 disposable o-rings
25 hybridization caps (single use)
50 disposable LifterSlip™ cover slips
CustomArray™
Stripping Kit
Product
Number
Stripping Kit for CustomArray™ 12K
610011
Stripping Kit for CustomArray™ 4x2K
610024
Required items to perform
CustomArray™ Stripping Protocol
CustomArray™ 12K or 4X2K
CustomArray™ Stripping Kit
CustomArray™ Stripping Clamp (re-usable up to
100 times)
The CustomArray™ Stripping Clamp
enables the CustomArray™ to be stripped and re-hybridized up to three
times. The Stripping Clamp is specially designed to withstand chemicals
used for stripping and to provide appropriate pressure to prevent
leakage from the stripping cap. The CustomArray™ Stripping
Clamp is recommended for up to 100 stripping procedures.
Product Number: 610010
If you have any questions or need assistance on
any of these products, please contact us at 1-800-985-CBMX or email
for more information.