The CombiMatrix MicroRNA product suite is built on
the CustomArray® 4X2K format. The catalog Species Specific MicroRNA
arrays consist of Human, Mouse, Rat, and Arabidopsis microarrays built
with probes from the Sanger
Database (version 13.0).
Species Specific MicroRNAs are listed below. To
see the probe list of any of the MicroRNAs, click on the link in the
table or on the photograph. For more information or to order a
microarray, click here.
*
Double controls included. CombiMatrix has excluded hairpins, therefore,
the number of MicroRNA probes is different from the Sanger database.
Species Specific MicroRNA Information:
Designs are based on unique microRNA
species from current release of Sanger database (Version 13.0 is at
http://microrna.sanger.ac.uk/sequences/)
Hairpin sequences are excluded from the
design process
Probes are anti-sense to mature microRNA
For each probe, a double mismatch control
is included to ensure specificity of detection. These ‘2mut’ controls
are designed to maximally de-stabilize probe-target hybridization,
thus, mutations are introduced in the two most stable triplets.
Non-microRNA controls include positive
controls corresponding to small RNA such as tRNA and U6. The small RNA
co-isolate with microRNA if the target preparation protocol is based on
size fractionation.
The negative controls contain anti-sense
probes designed for abundant mRNA (such as Actb (actin), HSPA4, PGK1).
If target samples have some RNA degradation, the partially digested
fragments of the abundant mRNA will co-isolate with microRNA during
size fractionation, and the corresponding probes will produce high
intensity signals. If target samples are of good quality, the negative
control signals will stay low.
MicroRNA Microarrays are compatible with
common reagents and scanners
All probes on every microRNA are 100%
quality tested
30 day turn-around time
Users can select the subset of organisms from the
Sanger 13.0 database for the Content Choice MicroRNA offered by
CombiMatrix. Users define the content as well as the controls on the
MicroRNA. Please contact us at
with questions.